Insect baculovirus expression vector system (BEVS) belongs to the eukaryotic expression system, and it’s an expression system with high safety. It has a large genome, which enables the insertion of large exogenous genes, therefore has the great advantage of expressing proteins with large molecular weight. It also has the ability to achieve complete post-translational modification and efficiently express exogenous genes. The system consists of transfer vector, baculovirus vector and the host cell. The system uses one or more baculovirus super-strong promoters, and gets the recombinant virus after the exogenous target gene is inserted into the promoter. The highly efficient expression of the exogenous gene is achieved while the recombinant viruses replicate themselves in the insect cells. BEVS is widely used in virus vaccine development (such as the development of influenza virus vaccine and HPV vaccine), preparation of cell signaling proteins and cytokines, as well as kinase development.
Abvigen can leverage the baculovirus expression system to support the production of difficult-to-express proteins, such as kinases, viral targets, cell surface receptors, transporters, various enzymes, and membrane proteins. Abvigen can express and purify membrane proteins while preserving their structural and functional integrity.
Codon optimization; gene synthesis
Ligation to expression vector
Obtain the correct recombinant plasmid
Preparation of recombinant Bacmid and high titer virus
Isolate recombinant bacmid DNA
Repeat the infection
Scale up expression and purification
Infect insect cells
Purification of target protein
Testing of purity, concentration, etc.
QC report is provided.
Short lead time
Cost-effective, high expression level
High High expression efficiency safety
After the two parties communicate and agree, determine the experimental plan, determine the service requirements - sign the contract - advance payment - start the experiment - deliver the results
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