Service Number：ABVH010
Introduction
Insect baculovirus expression vector system (BEVS) belongs to the eukaryotic expression system, and it’s an expression system with high safety. It has a large genome, which enables the insertion of large exogenous genes, therefore has the great advantage of expressing proteins with large molecular weight. It also has the ability to achieve complete post-translational modification and efficiently express exogenous genes. The system consists of transfer vector, baculovirus vector and the host cell. The system uses one or more baculovirus super-strong promoters, and gets the recombinant virus after the exogenous target gene is inserted into the promoter. The highly efficient expression of the exogenous gene is achieved while the recombinant viruses replicate themselves in the insect cells. BEVS is widely used in virus vaccine development (such as the development of influenza virus vaccine and HPV vaccine), preparation of cell signaling proteins and cytokines, as well as kinase development.
Custom Package
Abvigen can leverage the baculovirus expression system to support the production of difficult-to-express proteins, such as kinases, viral targets, cell surface receptors, transporters, various enzymes, and membrane proteins. Abvigen can express and purify membrane proteins while preserving their structural and functional integrity.
Description
Phase | Procedure | Timeline |
Plasmid construction | Codon optimization; gene synthesis Ligation to expression vector Obtain the correct recombinant plasmid | 2 weeks |
Preparation of recombinant Bacmid and high titer virus | Isolate recombinant bacmid DNA Obtain baculovirus Repeat the infection | 2 weeks |
Scale up expression and purification | Infect insect cells Purification of target protein | 1 week |
Quality Control | Testing of purity, concentration, etc. QC report is provided. | 1 week |
Advantages
Short lead time
Cost-effective, high expression level
Large capacity
High High expression efficiency safety
Service Process
After the two parties communicate and agree, determine the experimental plan, determine the service requirements - sign the contract - advance payment - start the experiment - deliver the results
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