Service Number£şABL031
Virus-like particles (VLPs) are non-infectious virus-derived structures made up of one or more different molecules with the ability to self-assemble, mimicking the form and size of a virus particle but lacking the genetic material. Like native viruses, specific epitopes displayed on VLPs can be taken up, processed, and presented by antigen-presenting cells to elicit potent specific humoral and cell-mediated immune responses. Therefore, VLPs is highly immunogenic and can induce humoral and cellular immune responses, and chimeric VLPs is used as a therapeutic vaccine.
The nanosized VLPs traffic through lymph nodes and are taken up by antigen-presenting cells (APCs). VLPs display diverse antigenic epitopes and induce both humoral and cellular anti-viral immune responses. Another advantage of VLPs is that it can be modified to display immunostimulatory cytokines as adjuvants to enhance the vaccine efficacy. These features suggest that VLPs can be used as a potent vaccine platform to develop. As an efficient vehicle, VLPs can deliver drugs to the targeted tissue for treatment or diagnostics. Thus, VLPs production is even more meaningful.
Abvigen offers the off shelf VLP productions and services to customize VLP generation and package the VLPs to mature ELISA kit, test standard reagent, and relevant products. Our experienced scientists have created a comprehensive platform to generate VLPs for both academic and industries.
Phase | Details | Timeline |
Preparation | Pool of 3 plasmids encoding viral structural proteins | N/A |
Bacterial Transformation | Vector construction Selection of Tranaformed Cells | 1-2 weeks |
Test Expression | Induce Determine Solubility SDS-PAGE | 1-2 weeks |
Scale Up | Induce Main culture | 1-2 week |
Isolation | NI-NTA Affinlty Chromatography | 1-2 week |
Purification | UV SDS-PAGE | 1-2 week |
Non-enveloped: Bud from the host cell without the host cell membrane.
Enveloped: Bud from the host cell carrying the host cell membrane and containing the viral proteins on the VLP surface.
Various systems have been utilized in the successful VLP construction, including bacterial, yeast, insect cells, plant and mammalian hosts.
Effectively express and purify membrane proteins.
Support a variety of VLP application services, such as cell targeting, biosensor, and chimeric VLP production.
A series of VLP characterization approaches, such as SRID, HA assay, DLS, and AFFFF-MALS.