The preparation of mouse immune sera is a rapid method to obtain polyclonal antibody production. We offer complete set of polyclonal antibody production, including antigen preparation, mice immunization, sera collection, and titer dectection, and antibody purification.
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We provide polyclonal antibody services from peptide synthesis, antigen production, antibody purification, to labeling. The specific service package is as follows.
Procedure
Procedure | Day | Description |
Pre-immune collection | 0 | 0.2-0.5 mL per mouse |
Injection | 1 | 0.10 mg antigen in FCA, SQ 4 sites |
1st booster | 21 | 50 μg antigen in FIA, SQ 4 sites |
2nd booster | 42 | 50 μg antigen in FIA, SQ 4 sites |
Test-bleed | 50 | 0.2-0.5 mL per mouse |
ELISA and shipping | 60 | ELISA titration of pre-immune and test-bleed |
3rd booster | 62 | 50 μg antigen in FIA, SQ 4 sites, verify disposition of mice |
Instruction due date | 80 | Terminal bleed (0.5-1 mL per mouse) |
Deliverables
15 vials crude antibody sera, including
5 vials: 0.1 mL test aliquots pre-immune sera
5 vials: 0.1 mL test bleeds post-inject sera
5 vials: 0.5-1 mL terminal bleeds
We guarantee to reach a minimum rabbit antiserum titer of 1:50,000 as measured with our ELISA assay.
Injections are subcutaneous as emulsions in FCA or FIA.