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Yeast Protein Expression Service

Cat No:ABVH008


Yeasts are outstanding hosts for the production of functional recombinant proteins with industrial or medical applications, and suitable for large-scale production of recombinant eukaryotic proteins. Yeast expression system provides the advantage on production of secreted & intracellular targets and post-translational modifications, which may be beneficial for some recombinant proteins. The common yeast expression hosts used for recombinant proteins expression include Saccharomyces cerevisiae, Pichia pastoris, Hansenula polymorpha, Yarrowia lipolytica, Arxula adeninivorans, Kluyveromyces lactis, and Schizosaccharomyces pombe. In Abvigen, various host cells and vectors are available for yeast expression.






Plasmid construction

Codon optimization; gene synthesis

Ligation to expression vector

Obtain the correct recombinant plasmid

2 weeks

Transformation and strain screening

Linearization of recombinant plasmid

Transform hosts by electroporation

Verify successful transformants by PCR

Obtain high copy

1-2 weeks

Small test

Small scale expression screening

2 weeks

Scale up expression and purification

Optimize expression conditions

Scale up culture

Protein purification

Quality Control

Testing of purity, concentration, etc. QC report is provided.

1 weeks



Proprietary Technologies: promoters, optimal copies of vector per cell, inducibility, signal peptides, and cellular location.

Humanized antibody production in yeast expression system.  

Streamlined Services: from gene synthesis, to the protein expression, antibody production, et al.


In addition, we also provide a variety of optional protein expression system, including

E. Coli ExpressionBaculovirus-Insect Expression
Mammalian ExpressionLarge-Scale Protein Production

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