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Stable Cell Line Construction Service

  • Overview
  • Service Details
  • Service Highlights
  • Related Services
  • Case Study
  • How to order
  • Overview

    Service Number£şABL010

    Cell line construction is the process of creating a large population of identical cells that contain the gene that, on translation, encodes the biologics (recombinant proteins, antibodies, fusion proteins, vaccines, etc.). When large quantities of recombinant protein are required, the construction of stable expression lines is recommended for production. Stable cell lines can be used for long-term continuous growth without significant changes in the expression levels of the target gene. The construction of stable cell line refers to the entry of foreign genes into recipient cells and integration into the chromosomes, allowing the target protein to be stably expressed on the surface of cell membrane. Stable cell line has been used in functional studies of genes, protein production and drug screening. Abvigen has been engaged in protein expression for many years and has extensive experience in developing stable cell lines, we use lentiviral vector for efficient gene delivery and to achieve high integration rate for several proteins, which can effectively enhance the production capacity. And we can provide customers with a one-stop service from gene sequence to stable cell line delivery.

    Service Details



    Cell Test

    Cell viability, cell density, and mycoplasma

    Construction of Lentiviral Expression Plasmid

    Codon optimization

    Gene synthesis

    Construction of lentiviral expression plasmid

    Lentivirus Packaging

    Co-transfer of expression plasmid and auxiliary plasmid into host cells

    Cell Pool Screening and Verification

    Positive cells screening 

    Detect cell pool expression level

    Single-Clone Screening and Verification

    Single cell planking and verification

    Quality Control

    Concentration; Purity; Contamination test

    Service Highlights

    Mature Stable Cell Line Development Platform

    Strict Quality Control System

    Professional Technical Support

    High yield and reproducibility

    High cell line stability

    Customizable Services




    Fusion protein





    Growth factor

    Related Services
    Case Study
    How to order