In the fields of molecular biology and pharmacology, the term small molecule refers to a low molecular weight (< 900 daltons) non-peptide, organic compound that may regulate a biological process. With the application of monoclonal antibody technology more and more widely, its production technology is becoming more and more perfect. In the recent rapid development of the molecular biosciences and their biotechnologicalapplications, and owing to their specific affinity, immunoassay systems using MAb against drugs andsmall molecular weight bioactive compounds have become an important tool for studies on receptorbinding analysis, enzyme assay, and quantitative and/or qualitative analytical techniques in animals orplants. The application of small molecules of drugs monoclonal antibody technology has been more and more widely, the technology of small molecule hapten carrier coupling and monoclonal antibody production provide strong technical support.
Abvigen can prepare various kinds of MAb against naturalproducts like solamarigine, crocin, marihuana compounds, opium alkaloids, ginsenosides, aconitine alkaloid, baicalin and so on, and developed a stable method as a sensitivity detection method.
Phase | Detail | Timeline |
Hapten conjugation | Small molecules compound conjugation to KLH and BSA | 1-2 weeks |
Antigen Purification | Antigen Purification Antigen QC | 1 weeks |
Immunization and serum titer test | Pre-immune bleed; 5-6 Balb/c mice immunization; Serum titer test | 10-12 weeks |
Fusion and screening | Fusion and Screening Subcloning and cell expansion | 10-12 weeks |
Functional screening (optional) | IC50 K value Cross-reactivity | 1-2 weeks |
Antibody production & purification | Appropriate scale hybridoma cell culture Purified by protein Aaffinity chromatography | 5-6 weeks |
Free antigen evaluation
Stable modification and coupling technology
Timely communication services
Reliable antibody purification technology
Complete experimental report
Free choice of poly/mono antibody
Various antibody modification