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ITRAQ/ TMT Labeling Quantitative Technique Service

  • Overview
  • Service Details
  • Service Highlights
  • Related Services
  • Case Study
  • How to order
  • Overview

    Service Number£ļABVH013

    Mass spectrometry has proven to be a key enabling technology for the quantification of changes in the proteome, surpassing the more traditional gel-based methods. Different methodologies have been applied, each with individual benefits and drawbacks. Broadly speaking, two experimental strategies currently prevail: label-based and label-free approaches. Chemical labelling using isobaric reagents (predominantly isobaric tags for relative and absolute quantification (iTRAQ) and tandem mass tags (TMT)) is widely applied in relative quantification workflows. The key features of iTRAQ and TMT are their multiplexing capability (currently 4-plex and 8-plex for iTRAQ and 6-plex for TMT) and the simultaneous identification and relative quantification of peptides and proteins. Such features are attractive and contribute to their popularity.

    Custom Package

    Abvigen iTRAQ/TMT technical services include protease digestion, peptide pricing, peptide separation, mass spectrometry analysis, mass spectrometry raw data analysis, bioinformatics analysis, etc.

    Service Details


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    Common sample types

    Serial No

    Type

    Use light

    1

    Organization

    > 200 mg

    2

    Cells

    1°Ń107

    3

    Protein

    > 500 ul

    >1 ug/ul

    4

    Co-IP

    2-5 ug

    5

    Blood

    > 1 ml


    Service Highlights

    Wide scope of application

    High throughput

    Reliable results

    High sensitivity

    Strong separation ability

    High degree of automation

    Service Process

    After the two parties communicate and agree, determine the experimental plan, determine the service requirements - sign the contract - advance payment - start the experiment - deliver the results

    Related Services
    Case Study
    How to order