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Mammalian Transient Protein Expression Service

  • Overview
  • Service Details
  • Service Highlights
  • Related Services
  • Case Study
  • How to order
  • Overview

    Service Number£şABL004

    More complex protein structures usually require particular post-translational modifications and co-factors to support their elaborate folding and enzymatic activity. E.coli expression system are unable to perform these modifications and lack essential eukaryotic co-factors. There appears to be a progressive increase in the application of mammalian cells for proteins production because of its highest level of protein processing, which is important for complete biological activity. The Overview of large-scale transient transfection has enabled the use of mammalian cells more attractive in terms of speed and ease of use, particularly for cell surface and secreted glycoproteins. Abvigen enables the high-throughput delivery and the generation high-quality of recombinant proteins and antibodies that are widely used in applications such as antigen preparation, antibody discovery, protein structure elucidation, and research for protein function.


    Service Details




    Plasmid construction

    Codon optimization;  gene synthesis

    Ligation to expression vector

    Obtain the correct recombinant plasmid

    2 weeks

    Small scale expression

    Transient transfect HEK293, CHO and other cells

    Detect expression products

    Optimization of transfection conditions

    1-2 weeks

    Scale up expression and purification

    Scale up the culture cells and transfect

    Optimal purification method

    1-2 weeks

    Quality Control

    Testing of purity, concentration, etc.  QC report is provided. 

    1 week

    Service Highlights

    Rapid expression of functional protein or antibody.

    Customization: quantity, purity, endotoxin, and other services are optional.

    Modifications: isotope labeling, protein biotinylation, and other services.

    Expression scale-up.

    Related Services
    Case Study
    How to order