Support:+1 929 202 3014


Fab/scFv Antibody Production Service

  • Overview
  • Service Details
  • Service Highlights
  • Related Services
  • Case Study
  • How to order
  • Overview

    Service Number£ºABL026

    Recombinant antibodies are now very important in both therapeutics and diagnostics and offer significant advantages over conventional antibodies because of their high specificity, sensitivity and stability, which may easily be optimised by genetic and chemical modification. There are various recombinant antibodies, including single chain variable fragment (scFv), fragment antigen-binding (Fab) region, single chain antibody fragment (scAb), bivalent scFv (sc(Fv)2), bivalent Fab region (F(ab¡ä)2) and scFv and Fab fusion (Fab-scFv). Single-chain fragment variables (scFv), is a combination of only the variable region of heavy chain (VH) and variable region of light chain (VL) domains. They are mainly constructed by three different methods: scFv generated from synthetic DNA, hybridoma technology or via accession number. A scFv (consisting of VH and V regions) is among the most popular recombinant antibody formats due to its small size, short generation time, capacity to be expressed in Escherichia coli (E. coli) and high antigen affinity and relative structural stability. 

    Abvigen is committed to provide advanced tools for scFv/Fab expression and purification. We have a series of well-tested bacterial expression systems established in house for high-yield and highly active scFv/Fab production. 

    Service Details




    Antibody sequence

    Antibody sequence

    Gene synthesis

    codon optimization

    1-2 weeks

    Vector construction

    Cloning into expression vectors

    Plasmid sequencing

    Plasmid production

    1-2 weeks

    Expression and purification

    Transient transfection of HEK293/CHO cells

    Antibody purification

    Polishing (optional)

    Transient transfection of HEK293/CHO cells

    Antibody purification

    Polishing (optional)

    QC analysis



    SEC-HPLC (optional)

    Endotoxin tests (optional)

    1 week

    Service Highlights

    Ensured reproducibility.

    Diverse fragment formats

    Different expression systems

    Secreting expression in periplasm of E. coli

    Related Services
    Case Study
    How to order