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Yeast Expression Service

  • Overview
  • Service Details
  • Service Highlights
  • Related Services
  • Case Study
  • How to order
  • Overview

    Service Number£şABL002

    Yeasts are outstanding hosts for the production of functional recombinant proteins with industrial or medical applications, and suitable for large-scale production of recombinant eukaryotic proteins. Yeast expression system provides the Highlights on production of secreted & intracellular targets and post-translational modifications, which may be beneficial for some recombinant proteins. The common yeast expression hosts used for recombinant proteins expression include Saccharomyces cerevisiae, Pichia pastoris, Hansenula polymorpha, Yarrowia lipolytica, Arxula adeninivorans, Kluyveromyces lactis, and Schizosaccharomyces pombe. In Abvigen, various host cells and vectors are available for yeast expression.


    Service Details
    Plasmid construction

    Codon optimization;   gene synthesis

    Ligation to expression vector

    Obtain the correct recombinant plasmid

    2 weeks
    Transformation and strain screening

    Linearization of recombinant plasmid

    Transform hosts by electroporation

    Verify successful transformants by PCR

    Obtain high copy

    1-2 weeks
    Small test

    Small scale expression screening

    2 weeks

    Scale up expression and purification

    Optimize expression conditions

    Scale up culture

    Protein purification

    Quality Control

    Testing of purity, concentration, etc. QC report is provided.

    1 weeks

    Service Highlights

    Proprietary Technologies: promoters, optimal copies of vector per cell, inducibility, signal peptides, and cellular location.

    Humanized antibody production in yeast expression system.  

    Streamlined Services: from gene synthesis, to the protein expression, antibody production, et al.

    Related Services
    Case Study
    How to order