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Mouse Monoclonal Antibody Production Service

  • Overview
  • Service Details
  • Service Highlights
  • Related Services
  • Case Study
  • How to order
  • Overview

    A mouse monoclonal antibody is a type of antibody produced by a single clone of immune cells derived from mice. After immunization, antibody-secreting B cells are immortalized by fusion to myeloma cell lines, and the resultant hybridomas are screened using a client-directed customizable protocol to select for the desired antigen-specific antibody. Mouse immunization and spleen fusion to create hybridoma cell lines are two phases of an entire procedure for developing, producing, and purifying monoclonal antibodies.

    Abvigen's custom mouse monoclonal antibody development service provides a comprehensive solution for generating high-quality monoclonal antibodies against any target of interest. The service includes immunogen design and synthesis, immunization, hybridoma generation, screening, and antibody production.

    We perform and report in-process ELISA testing and send samples to customers for evaluation, allowing customers to select which animals to use for fusion and which cell lines to subclone, expand, and deliver.

    1Pre sales communication.jpg2 Receiving orders.jpg3 gene synthesisvector construction.jpg
    4 Protein expression Peptide Synthesis.jpg5 Animal immunity.jpg6 Antiserum testing.jpg
    7 antibody purification.jpg8 Antibody validation.jpg9 Antibody delivery.jpg

    Service Details




    Antigen preparation

    Antigen designn

    Antigen synthesis and expression

    Abvigen provided: 1-2 business days

    Peptide synthesis: 2-3 weeks

    Protein Expression: 2-4 weeks

    Immunization and ELISA titration

    Conventional or accelerated immunization protocol

    ELISA Titer testing

    9-10 weeks

    Fusion and screening

    Fusion clone screening by indirect ELISA

    Addition screening assays available upon request

    4-5 weeks

    Subcloning before production

    Subclone screening

    6-7 weeks

    Production and purification

    Small-scale antibody purification

    Isotype identification

    Additional screening and characterization assays available upon request

    Antibody purification

    Antibody validation by indirect ELISA

    Additional validation, scale-up, and antibody engineering available upon request

    Low-endotoxin and serum-free culturing are available on request, as well as high-volume production

    5-6 weeks


    2-5 ELISA positive clones

    Purified antibodies, 0.1-0.5 mg/clone

    Remaining Antigens

    Final Project Report


    We offer a wide variety of Custom Antibody Labeling Services options

    1-2 weeks

    *Immunogen type: protein, peptides, small molecules, DNA, mRNA, virus-like particles, cell lines and etc.

    Service Highlights

    Advanced antibody discovery platforms

    Cutting-Edge Technology & Facility

    Well-established generation platforms

    Various Antibody Production Technologies

    A wide range of antigens

    Antibody validation approaches

    Expert-guided custom project design

    Related Services
    Case Study
    How to order