Service Number：ABVH009
Introduction
The prokaryotic expression system has the advantages of high expression level, simple operation, short cycle, easy large-scale and high-density culture and low cost. For the full-length antibody and glycoprotein biological drug, the folding of expression product polypeptide chain, the disulfide bond, the presence or absence of glycosylation and the type of glycosylation often affect the properties of the synthesis, secretion, biological activity, in vivo stability, and immunogenicity of the expressed product. Compared with other eukaryotic expression systems, the expression of the target gene in mammalian cells is similar to that of the native protein in the type and manner of the glycosylation, and can be correctly assembled into the multi-subunit protein.
Custom Package
Abvigen enables the high-throughput delivery and the generation high-quality of recombinant proteins and antibodies that are widely used in applications such as antigen preparation, antibody discovery, protein structure elucidation, and research for protein function.
Description
Phase | Procedure | Timeline |
Plasmid construction | Codon optimization; gene synthesis Ligation to expression vector Obtain the correct recombinant plasmid | 2 weeks |
Small scale expression | Transient transfect HEK293, CHO and other cells Detect expression products Optimization of transfection conditions | 1-2 weeks |
Scale up expression and purification | Scale up the culture cells and transfect Optimal purification method | 1 week |
Quality Control | Testing of purity, concentration, etc. QC report is provided | 1 week |
Advantages
Short lead time
No self-produced endotoxin
Cost-effective, high expression level
Products can be properly folded and efficient secretion
With a variety of complex N-linked glycosylation, accurate O-linked glycosylation and other post-translational processing
Multi-cell lines, multi-expression methods to improve the protein expression success rate and protein yield
Service Process
After the two parties communicate and agree, determine the experimental plan, determine the service requirements - sign the contract - advance payment - start the experiment - deliver the results
More Production Services
Protein Antibody Sequencing Service
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