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Services

Mammalian Cell Expression Service

Service NumberABVH009

Introduction

The prokaryotic expression system has the advantages of high expression level, simple operation, short cycle, easy large-scale and high-density culture and low cost. For the full-length antibody and glycoprotein biological drug, the folding of expression product polypeptide chain, the disulfide bond, the presence or absence of glycosylation and the type of glycosylation often affect the properties of the synthesis, secretion, biological activity, in vivo stability, and immunogenicity of the expressed product. Compared with other eukaryotic expression systems, the expression of the target gene in mammalian cells is similar to that of the native protein in the type and manner of the glycosylation, and can be correctly assembled into the multi-subunit protein.

 

Custom Package

Abvigen enables the high-throughput delivery and the generation high-quality of recombinant proteins and antibodies that are widely used in applications such as antigen preparation, antibody discovery, protein structure elucidation, and research for protein function.

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Description

 

Phase

Procedure

Timeline

Plasmid construction

Codon optimization; gene synthesis

Ligation to expression vector

Obtain the correct recombinant plasmid

2 weeks

Small scale expression

Transient transfect HEK293, CHO and other cells

Detect expression products

Optimization of transfection conditions

1-2 weeks

Scale up expression and purification

Scale up the culture cells and transfect

Optimal purification method

1 week

Quality Control

Testing of purity, concentration, etc. QC report is provided

1 week

 

Advantages

Short lead time

No self-produced endotoxin

Cost-effective, high expression level

Products can be properly folded and efficient secretion

With a variety of complex N-linked glycosylation, accurate O-linked glycosylation and other post-translational processing

Multi-cell lines, multi-expression methods to improve the protein expression success rate and protein yield

 

Service Process

After the two parties communicate and agree, determine the experimental plan, determine the service requirements - sign the contract - advance payment - start the experiment - deliver the results

  

More Production Services

Protein Antibody Sequencing Service

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