Support:+1 929 202 3014


E. Coli Protein Expression Service

  • Overview
  • Service Details
  • Service Highlights
  • Related Services
  • Case Study
  • How to order
  • Overview

    Service Number£şABL001

    Escherichia coli expression system continues to dominate the bacterial expression systems and remain to be the preferred system.  Escherichia coli expression system is is regarded as classical expression system because of its simple structure, successful heterologous gene expression, short culture period and high level protein expression.  How to optimize soluble protein expression in E. coli, that is the focus and Highlights of our company.  Abvigen has extensive experience and be very professional in E. coli protein expression and purification.  We can solve various problems in the process of protein expression to meet the needs of customers.


    Service Details




    Plasmid construction

    Codon optimization;  gene synthesis

    Ligation to expression vector

    Obtain the correct recombinant plasmid

    2 weeks

    Transformation and strain screening

    Transform the recombinant plasmid to host cells

    Select single colony for small-scale induced expression

    Optimize the expression condition 

    1 week

    Target protein expression and purification

    Large-scale expression

    Protein purification

    1 week

    Inclusion body renaturation

    Refolding if the target protein is inclusion body

    1 week

    Quality Control

    Testing of purity, concentration, etc.  QC report is provided. 

    1 week

    Service Highlights

    Guaranteed yield: 1-3 mg purified protein.

    Guaranteed purity: >85% protein purity.

    Soluble protein: we prioritize proteins obtained from supernatant.

    Rich experience: many kinds of protein purification and protein regenerating experience

    High success rate: the success rate of expression is higher than 95%

    Related Services
    Case Study
    How to order