Service Number£şABL001
Escherichia coli expression system continues to dominate the bacterial expression systems and remain to be the preferred system. Escherichia coli expression system is is regarded as classical expression system because of its simple structure, successful heterologous gene expression, short culture period and high level protein expression. How to optimize soluble protein expression in E. coli, that is the focus and Highlights of our company. Abvigen has extensive experience and be very professional in E. coli protein expression and purification. We can solve various problems in the process of protein expression to meet the needs of customers.
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Phase | Procedure | Timeline |
Plasmid construction | Codon optimization; gene synthesis Ligation to expression vector Obtain the correct recombinant plasmid | 2 weeks |
Transformation and strain screening | Transform the recombinant plasmid to host cells Select single colony for small-scale induced expression Optimize the expression condition | 1 week |
Target protein expression and purification | Large-scale expression Protein purification | 1 week |
Inclusion body renaturation | Refolding if the target protein is inclusion body | 1 week |
Quality Control | Testing of purity, concentration, etc. QC report is provided. | 1 week |
Guaranteed yield: 1-3 mg purified protein.
Guaranteed purity: >85% protein purity.
Soluble protein: we prioritize proteins obtained from supernatant.
Rich experience: many kinds of protein purification and protein regenerating experience
High success rate: the success rate of expression is higher than 95%