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Phospho-Specific Monoclonal Antibody Production Service

  • Overview
  • Service Details
  • Service Highlights
  • Related Services
  • Case Study
  • How to order
  • Overview

    Service Numberú║ABL019

    The original antibodies are susceptible to degradation in vivo by factors such as proteases, oxidants, and heat. By introducing modified groups, we can enhance the stability of the antibodies and prolong their half-life in the body, thereby increasing their therapeutic efficacy and persistence. These states of protein expression and activation include phosphorylation, acetylation, glycosylation, prenylation, myristoylation, ubiquitination, sumoylation, protein cleavage (neo-epitopes), splice variants, and isoforms. 

    However, the process of producing antibodies against a phospho-residue is more complicated than using peptide immunogens. Phospho-specific antibodies are generated using peptides containing one or more phosphorylated amino acids. There are three residues which can be phosphorylated: Serine (S), Threonine (T) and Tyrosine (Y). 

    Abvigen provides a custom phospho-specific antibody production service. Typically such a project is a monoclonal anti-peptide antibody. We can use the Phospho-Peptides to immunize the mice, and use Phospho-Peptides to screen for positive hybridoma clones, and use non-phospho-peptides to do negative selection. This negative selection is required since peptide phosphorylation or protein phosphorylation is never 100% complete.


    1Pre sales communication.jpg

    2 Receiving orders.jpg

    3 gene synthesisvector construction.jpg

    4 Protein expression Peptide Synthesis.jpg

    5 Animal immunity.jpg

    6 Antiserum testing.jpg

    7 antibody purification.jpg

    8 Antibody validation.jpg

    9 Antibody delivery.jpg

    Service Details

    Phase

    Details

    Timeline

    Antigen Preparation

    Peptide design, synthesis and conjugation

    Synthesis of phospho immunisation peptide (10 mg, high purity) and a non-phospho control peptide.

    Conjugation of the immunisation peptide to KLH or BSA

    Control peptide (optional)

    1-2 weeks

    Immunization

    Immunize 5-10 Balb/c mice

    Conventional or accelerated immunization protocol

    ELISA Titer testing

    8-10 weeks

    Screening and Subcloning

    Fusion and ELISA screening

    Subcloning (2-4 rounds)

    2-4 weeks

    Antibody Production & 

    Purification

    Hybridoma cell culture

    Antibody production

    Phospho specific affinity purification

    1-2 weeks

    Validation and Delivery

    Evaluate product quality: SDS-PAGE

    Shipping

    1-2 weeks


    Service Highlights

    High specificity 

    ELISA positive with phospho peptide

    Low cross-reactivity

    Low price with fast turnaround

    One-step service


    Related Services
    Case Study
    How to order