Introduction
Escherichia coli expression system continues to dominate the bacterial expression systems and remain to be the preferred system. Escherichia coli expression system is is regarded as classical expression system because of its simple structure, successful heterologous gene expression, short culture period and high level protein expression. How to optimize soluble protein expression in E. coli, that is the focus and advantage of our company. Abvigen has extensive experience and be very professional in E. coli protein expression and purification. We can solve various problems in the process of protein expression to meet the needs of customers.
Description
Phase | Procedure | Timeline |
Plasmid construction | Codon optimization; gene synthesis Ligation to expression vector Obtain the correct recombinant plasmid | 2 weeks |
Transformation and strain screening | Linearization of recombinant plasmid Transform hosts by electroporation Verify successful transformants by PCR Obtain high copy | 1-2 weeks |
Small test | Small scale expression screening | 2 weeks |
Scale up expression and purification | Optimize expression conditions Scale up culture Protein purification | |
Quality Control | Testing of purity, concentration, etc. QC report is provided. | 1 weeks |
Advantage
Guaranteed yield: 1-3 mg purified protein.
Guaranteed purity: >85% protein purity.
Soluble protein: we prioritize proteins obtained from supernatant.
Rich experience: many kinds of protein purification and protein regenerating experience
High success rate: the success rate of expression is higher than 95%
In addition, we also provide a variety of optional protein expression system, including
| Yeast Expression Service | Baculovirus-Insect Expression Service |
| Mammalian Expression Service | Large-Scale Protein Production Service |
Online Inquiry
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