Service Number：ABVH007
Introduction
Escherichia coli is one of the most widely used hosts for the production of heterologous proteins and its genetics are far better characterized than those of any other microorganism. The E. coli expression system is regarded as the most commonly used, economical, and classical expression system because of its simple structure, clear genetic background, high yield of target protein, and its short culture period. In recent decades, E. coli expression system has also been developed and improved continuously, and been used intensively by scientific researchers and industrial users for a large number of recombinant protein expression. The system is mainly used for antigen preparation, ligand preparation, and expression of cytokines and bacteria (Staphylococcus aureus, Escherichia coli, etc.) proteins services.
Custom Package
Abvigen has extensive experience and be very professional in E. coli protein expression and purification. We can solve various difficult problems during the protein expression and purification process. We have successfully developed many proteins expressed in E. coli, which contain proteins with high purity.
Description
Phase | Procedure | Timeline |
Plasmid construction | Codon optimization; gene synthesis Ligation to expression vector Obtain the correct recombinant plasmid | 2 weeks |
Transformation and strain screening | Transform the recombinant plasmid to host cells Select single colony for small-scale induced expression Optimize the expression conditions | 1 week |
Target protein expression and purification | Large-scale expression Protein purification | 2 weeks |
Inclusion body renaturation | Refolding if the target protein is inclusion body | |
Quality Control | Testing of purity, concentration, etc. QC report is provided. | 1 weeks |
Advantages
Short lead time
Clear genetic background
Fast growth and high yield
Cost-effective, easy for large-scale production
More options for vectors and tags, higher success rate
Easy to optimize various conditions to achieve the best results
Service Process
After the two parties communicate and agree, determine the experimental plan, determine the service requirements - sign the contract - advance payment - start the experiment - deliver the results
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