Escherichia coli expression system continues to dominate the bacterial expression systems and remain to be the preferred system. Escherichia coli expression system is is regarded as classical expression system because of its simple structure, successful heterologous gene expression, short culture period and high level protein expression. How to optimize soluble protein expression in E. coli, that is the focus and advantage of our company. Abvigen has extensive experience and be very professional in E. coli protein expression and purification. We can solve various problems in the process of protein expression to meet the needs of customers.
Codon optimization; gene synthesis
Ligation to expression vector
Obtain the correct recombinant plasmid
Transformation and strain screening
Linearization of recombinant plasmid
Transform hosts by electroporation
Verify successful transformants by PCR
Obtain high copy
Small scale expression screening
Scale up expression and purification
Optimize expression conditions
Scale up culture
Testing of purity, concentration, etc. QC report is provided.
Guaranteed yield: 1-3 mg purified protein.
Guaranteed purity: >85% protein purity.
Soluble protein: we prioritize proteins obtained from supernatant.
Rich experience: many kinds of protein purification and protein regenerating experience
High success rate: the success rate of expression is higher than 95%
In addition, we also provide a variety of optional protein expression system, including
|Yeast Expression Service
|Baculovirus-Insect Expression Service
|Mammalian Expression Service
|Large-Scale Protein Production Service