Conjugate antibodies, proteins, peptides or other biomolecules
Description:
Amine Magnetic Particles: 1 mL
Coupling Buffer (CB300): 15 mL
Quenching Buffer (QB300): 0.5 mL
Storage Buffer: 15 mL
Sulfo-SMCC: 20 mg (Store at -20 °C upon arrival)
Diameter:
200 nm
Functional Group:
Carboxyl
Storage:
EDC and Sulfo-NHS -20 °C; Others 2-8 °C
General Information
Background:
Carboxyl magnetic beads of various diameters are now available in an easy-to-use kit format to enable researchers to conjugate antibodies, proteins, peptides or other biomolecules of their own choice to these magnetic beads. Attribute to the large surface area, excellent colloidal stability and unique surface coating of the Carboxyl Beads as well as the specifically formulated conjugation buffers, the resulting conjugates exhibit high binding capacity and low non-specific binding. The kit contains sufficient reagents & components for performing at least 4 conjugation reactions. Briefly, the magnetic beads are activated using carbodiimide and N-hydroxysuccinimide followed by conjugation to amino groups that are present on the target protein/ligand. The recommended protocol has been used to successfully conjugate bovine serum albumin, streptavidin or immunoglobulin to these magnetic beads.