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Nucleic Acid Labeling Service

Service NumberABVH019

Introduction

In order to capture a single DNA or RNA chain, determine the existence of transcripts of target genes or monitor gene expression, and observe their exact functions, nucleic acid labeling technology provides researchers with a powerful diagnostic tool.

 

Custom Package

Abvigen launched the nucleic acid labeling technology service platform to covalently bind common probes labeled with enzymes, biotins and fluorescent chemical groups to DNA or RNA through chemical reaction steps. The combination of reagents and nucleic acid molecules will not change the structure of nucleic acids, nor will it affect downstream hybridization applications. The labeled nucleic acid molecules can be used in a variety of experiments according to the needs of researchers.

 

Description

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Probe

Enzyme; TAMRA; Dabcyl

Hapten

Biotin; Digoxin

Fluorescein

FITC; CyCy3Cy5Cy5.5Cy7

Chemical dye

CAL FluorROX

Colloidal gold label

Co-Au

 

 


Method

Reagent

Mark

Probe

Site

DNA

Enzyme

5-

T4 PNK

γ-32PrATP

ssDNA

5-OH

3-

TdT

dNTP

ssDNA

3-OH

Nick Translation

DNA-pol I

dNTP

DNA

Random

Primer Extension

Kelnow

dNTP

DNA

Random

PCR

Taq Polymerase

dNTP

DNA

Random

Chemical

Conjugate

EDC

Amines/Carboxylates/Phosphates

DNA

5-OH

Photoreaction

Nonspecific cross linker

Nucleobase

DNA

Random

RNA

Enzyme

Reverse Transcription

Reverse Transcriptase

dNTP

cDNA

Random

5-

T4 PNK

γ-32PrATP

RNA

5-OH

3-

T4 RNA Ligase

[5-32P]pCp

RNA

3-OH

Chemical

Oxidation

Periodate

Amine/Hydrazide

RNA

3-OH

Conjugate

EDC

Amines/Carboxylates/Phosphates

RNA

5-OH

Photoreaction

Nonspecific cross linker

Nucleobase

RNA

Random

 

Advantage

Stable

Well Characterized

Quick turnaround

 

Service Process

After the two parties communicate and agree, determine the experimental plan, determine the service requirements - sign the contract - advance payment - start the experiment - deliver the results

  

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