Membrane proteins are the molecular interface between host and pathogen, membrane proteins embedded within the cellular membrane serve a critical purpose in the maintenance of many cellular functions.Antibodies are essential for structural determinations and functional studies of membrane proteins. Target-specific monoclonal antibodies have allowed the determination of novel membrane protein structures during electron cryomicroscopy by increasing the size of the target, and during crystallography by stabilizing unique protein conformations. Given the importance in cellular communication and transportation of molecules and ions across the cell membrane, membrane proteins become the largest category of druggable targets studied in the pharmaceutical industry. However, antibody generation is limited by the availability of properly-folded and purified antigen.
Therefore, we have provided several multiple transmembrane protein antibody preparation method, including the classic soluble zone protein design scheme, recombinant protein immunization scheme, and custom service of antibody preparation.
Biotyscience can provide many protocols of membrane protein antibody production for customers, we can also provide customers with one-stop membrane protein antibody production services from peptide design, immunization, to labeling and purification.
Phase | procedure | Time |
Peptide design and synthesis | Peptide design, peptide synthesis, conjugation (KLH, BSA) | 2 weeks |
Protein preparation | cDNA synthesis, Vector construction, protein expression & purification | 6-8 weeks |
Immunization and Test | Immunized 3-5 mice/2 rabbits, ELISA detection | 4-6 weeks |
Fusion | Isolating Spleen cells, B cells+Myeloma cell, get fused cells (hybridomas) | 1-2 weeks |
Proliferation and screening | Selection in HAT medium | 1-2 weeks |
Growing cells/subcloning | Positive test, and cloning producing the desired antibody | 1-2 weeks |
Ascites producion/culture in vitro | Mass production of monoclonal antibodies | 3-4 weeks |
Purification & detection | Affinity purification/Antigen specific affinity purification | 2 weeks |
3-5 ml Pre-immune sera
15-20 ml antiserum
Purified antibodies
Complete project report
Monoclonal antibody preparation service Antibody labeling service
Recombinant antibody expression service Other custom services
Abvigen committed to providing one-stop antibody customization services from antigen synthesis to antibody manufacturing for scientific research institutions and pharmaceutical enterprises. Our technical team will rely on rich technical experience to serve you wholeheartedly.
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