Service Number：ABVH002
Introduction
Single B cell isolation offers a fast, high-throughput method to generate a cell line producing your antibody of interest. B cells are isolated from an immunized animal, sorted into single wells, lysed, and their genomes sequenced. Genes encoding desired proteins are cloned into a vector and transiently transfected into mammalian cells. Utilize a single B cell workflow to leapfrog many of the traditional antibody engineering challenges, such as long turnaround time, extensive hands-on inputs, and multiple rounds of cell culture.
Custom Package
Abvigen has been committed to producing high-quality antibody services. The antibody development platform based on single B cell technology can directly obtain positive B cells from peripheral blood and clone antibody gene sequences after animal immunization. Thousands of target binding positive B cell clones can be obtained, which can more efficiently screen antibodies with high specificity, high sensitivity and high affinity.
Description
Phase | Procedure | Timeline |
Animal Immunity | Immunized 3-5 mice/BALB/c mice ELISA detection | 6-8 weeks |
B-Cell Harvest | Plasma Cell enrichment Single Cell Sorting | 2-3 weeks |
Single B-Cell Sorting Colony Picking | Multiple RT-PCR Sequencing | 3-4 weeks |
Vector Build and Transfection | Antibody Expression | 3-4 weeks |
Purification & detection | SDS-PAGE ELISA SEC-HPLC | 2 weeks |
Advantages
High drug resistance
No species restriction
Many positive clones
Complete services
Service Process
After the two parties communicate and agree, determine the experimental plan, determine the service requirements - sign the contract - advance payment - start the experiment - deliver the results
More Production Services
Small-molecules monoclonal antibody Preparation
Acetylated monoclonal antibody preparation
Methylated monoclonal antibody preparation
Membrane Protein Antibody Production
Online Inquiry
To learn more about information protection, please see Privacy Policy. If you have any questions about CRO service, please email at info@abvigenus.com or call 929-202-3014