Service Number:ABVH002
Introduction
Single B cell isolation offers a fast, high-throughput method to generate a cell line producing your antibody of interest. B cells are isolated from an immunized animal, sorted into single wells, lysed, and their genomes sequenced. Genes encoding desired proteins are cloned into a vector and transiently transfected into mammalian cells. Utilize a single B cell workflow to leapfrog many of the traditional antibody engineering challenges, such as long turnaround time, extensive hands-on inputs, and multiple rounds of cell culture.
Custom Package
Abvigen has been committed to producing high-quality antibody services. The antibody development platform based on single B cell technology can directly obtain positive B cells from peripheral blood and clone antibody gene sequences after animal immunization. Thousands of target binding positive B cell clones can be obtained, which can more efficiently screen antibodies with high specificity, high sensitivity and high affinity.
Description
Phase | Procedure | Timeline |
Animal Immunity | Immunized 3-5 mice/BALB/c mice ELISA detection | 6-8 weeks |
B-Cell Harvest | Plasma Cell enrichment Single Cell Sorting | 2-3 weeks |
Single B-Cell Sorting Colony Picking | Multiple RT-PCR Sequencing | 3-4 weeks |
Vector Build and Transfection | Antibody Expression | 3-4 weeks |
Purification & detection | SDS-PAGE ELISA SEC-HPLC | 2 weeks |
Advantages
High drug resistance
No species restriction
Many positive clones
Complete services
Service Process
After the two parties communicate and agree, determine the experimental plan, determine the service requirements - sign the contract - advance payment - start the experiment - deliver the results
More Production Services
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Online Inquiry
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