Introduction
Cell line construction is the process of creating a large population of identical cells that contain the gene that, on translation, encodes the biologics (recombinant proteins, antibodies, fusion proteins, vaccines, etc.). When large quantities of recombinant protein are required, the construction of stable expression lines is recommended for production. Stable cell lines can be used for long-term continuous growth without significant changes in the expression levels of the target gene. The construction of stable cell line refers to the entry of foreign genes into recipient cells and integration into the chromosomes, allowing the target protein to be stably expressed on the surface of cell membrane. Stable cell line has been used in functional studies of genes, protein production and drug screening. Abvigen has been engaged in protein expression for many years and has extensive experience in developing stable cell lines, we use lentiviral vector for efficient gene delivery and to achieve high integration rate for several proteins, which can effectively enhance the production capacity. And we can provide customers with a one-stop service from gene sequence to stable cell line delivery.
Description
Phase | Procedure |
Cell Test | Cell viability, cell density, and mycoplasma |
Construction of Lentiviral Expression Plasmid | Codon optimization Gene synthesis Construction of lentiviral expression plasmid |
Lentivirus Packaging | Co-transfer of expression plasmid and auxiliary plasmid into host cells |
Cell Pool Screening and Verification | Positive cells screening Detect cell pool expression level |
Single-Clone Screening and Verification | Single cell planking and verification |
Quality Control | Concentration; Purity; Contamination test |
Application
Antibody
Enzyme
Fusion protein
Bispecific
Biosimilars
Cytokine
Hormones
Growth factor
Advantage
Mature Stable Cell Line Development Platform
Strict Quality Control System
Professional Technical Support
High yield and reproducibility
High cell line stability
Customizable Services
In addition, we also provide a variety of optional peptide Synthesis and analysis, including
Online Inquiry
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