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Endotoxin-Free Protein Production Service

Cat No:ABL009


Endotoxins, known as lipoglycans and lipopolysaccharides (LPS), are a part of the outer membrane of the cell wall of Gram-negative bacteria. It is extremely hard to remove them by simple sterilization. Endotoxins can initiate a strong immune response such as endotoxin shock, organ failure, tissue injury, et al. Due to the harmful effects of endotoxin, limiting the protein endotoxin level has become an important direction in the medical field. E. Coli is a preferred system for recombinant protein because it generally provides the most cost effective solution. However, there are several limitations, include difficultly in production for proteins with strict endotoxin condition requirements and poor secretion for secretory proteins. To break out the limit, Abvigen has developed endotoxin-free Expression System, which is able to express a wide variety of protein and oligonucleotides as the wild-type counterparts except that they lack endotoxic activity.





Gene synthesis

Codon optimization; gene synthesis

Protein expression & purification

Transformation and strain screening

Target protein expression

Protein purification

Endotoxin removal

Endotoxin affinity chromatography

Ion exchange chromatography

Hydrophobic chromatography

Gel filtration chromatography and ultrafiltration

Triton X-114 phase separation

Endotoxin detection

LAL gel-clot assay

LAL turbidimetric assay

LAL chromogenic assay



In addition, we also provide a variety of optional peptide Synthesis and analysis, including

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