Conjugate quantum dots and antibodies, proteins, peptides or other biomolecules with primary amine, carboxy or thiol groups with low non-specific binding
Description:
NHS Beads: 10 mg
Coupling Buffer: 15 mL
Queching Buffer: 1 mL
Storage Buffer: 30 mL
Diameter:
525 nm
Functional Group:
Amine
Storage:
Sulfo-SMCC -20 °C; Others 2-8 °C
General Information
Background:
Quantum dots are fluorescent semiconductor nanocrystals that have unique optical and electronic properties including size tunable emission wavelength, superior brightness, high resistance to photobleaching, and simultaneous excitation of multiple colors from a single light source. These properties make QDs a unique class of light-emitting nanoparticles with promising potential in optoelectronics, biotechnology and medicine. Amine-terminated quantum dots, ranging from 425-665 nm, are now available in an easy-to-use kit format , allowing researchers to conjugate antibodies, proteins, peptides or other biomolecules of their own choice. The kit is used to specifically conjugate quantum dots and antibodies, proteins, peptides or other biomolecules with primary amine, carboxy or thiol groups with low non-specific binding. It contains Amine Quantum Dots and reagents sufficient for performing four conjugation reactions. Briefly, the QDs are activated using SMCC cyclohexane-1-carboxylate), followed by conjugation to thiol groups that are present on the target protein/ligands such as bovine serum albumin, streptavidin and immunoglobulin.
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