Protein phosphorylation represents an approach to study the molecular basis for a wide variety of neurophysiological phenomena. Protein phosphorylation is a significant post-translational modification (PTM) in cells, and plays an important role in cell signaling and apoptosis process, and it is the activity regulatory center to maintain cell life by regulating protein biological activity, including activation or inhibition of protein activity, thereby further regulating the cellular signal pathway. The phosphorylation binding sites are different in eukaryotes (serine, threonine and tyrosine) and prokaryotes (histidine). Nowadays Phosphorylated antibodies are an important tool to study protein phosphorylation.
Abvigen can provide many protocols of phosphorylated antibody production for customers, we can also provide customers with one-stop phosphorylated antibody validation services, including phosphorylation and non-phosphorylated antigen cross-reactivity, Western Blot, and immunohistochemistry.
Phase | procedure | Time |
Phosphorylated polypeptides design & synthesis | Peptide design, Peptide synthesis, conjugation (KLH, BSA) | 2 weeks |
Protein preparation | Vector construction & purification | 2-3 weeks |
Immunization and Test | Immunized 3-5 mice, ELISA detection | 4-6 weeks |
Fusion | Isolating Spleen cells, B cells+Myeloma cell, get fused cells (hybridomas) | 1-2 weeks |
Proliferation and screening | Selection in HAT medium, well plate with antigen bound to surface, test for binding to antigen | 1-2 weeks |
Growing cells/subcloning | Positive test, and cloning producing the desired antibody | 1-2 weeks |
Ascites producion/culture in vitro | Mass production of monoclonal antibodies | 3-4 weeks |
Purification & detection | Affinity purification/Antigen specific affinity purification, ELISA detection | 2 weeks |
(refer to DETAI and AB)
5mg Modified polypeptide
3mg Unmodified polypeptides and antibody products
preimmune sera
2-5 strains of hybria cells
3-8mg purified antibodies
Complete project report
Monoclonal antibody preparation service Antibody labeling service
Recombinant antibody expression service Other custom services
Abvigen is committed to providing one-stop antibody customization services from antigen synthesis to antibody manufacturing for scientific research institutions and pharmaceutical enterprises. Our technical team will rely on rich technical experience to serve you wholeheartedly.
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