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Phosphorylated Monoclonal Antibody Production



Protein phosphorylation represents an approach to study the molecular basis for a wide variety of neurophysiological phenomena. Protein phosphorylation is a significant post-translational modification (PTM) in cells, and plays an important role in cell signaling and apoptosis process, and it is the activity regulatory center to maintain cell life by regulating protein biological activity, including activation or inhibition of protein activity, thereby further regulating the cellular signal pathway. The phosphorylation binding sites are different in eukaryotes (serine, threonine and tyrosine) and prokaryotes (histidine). Nowadays Phosphorylated antibodies are an important tool to study protein phosphorylation.


Custom Package   

Abvigen can provide many protocols of phosphorylated antibody production for customers, we can also provide customers with one-stop phosphorylated antibody validation services, including phosphorylation and non-phosphorylated antigen cross-reactivity, Western Blot, and immunohistochemistry.







Phosphorylated polypeptides  design & synthesis

Peptide design, Peptide synthesis, conjugation (KLH, BSA)

2 weeks

Protein preparation

Vector construction & purification

2-3 weeks

Immunization and Test

Immunized 3-5 mice, ELISA detection

4-6 weeks


Isolating Spleen cells, B cells+Myeloma cell, get fused cells (hybridomas)

1-2 weeks

Proliferation and screening

Selection in HAT medium, well plate with antigen bound to surface, test for binding to antigen

1-2 weeks

Growing cells/subcloning

Positive test, and cloning producing the desired antibody

1-2 weeks

Ascites producion/culture in vitro

Mass production of monoclonal antibodies

3-4 weeks

Purification & detection

Affinity purification/Antigen specific affinity purification, ELISA detection

2 weeks


(refer to DETAI and AB)



5mg Modified polypeptide

3mg Unmodified polypeptides and antibody products

preimmune sera

2-5 strains of hybria cells

3-8mg purified antibodies

Complete project report


More Production Services

Monoclonal antibody preparation service       Antibody labeling service

Recombinant antibody expression service      Other custom services


Get a Quote

Abvigen is committed to providing one-stop antibody customization services from antigen synthesis to antibody manufacturing for scientific research institutions and pharmaceutical enterprises. Our technical team will rely on rich technical experience to serve you wholeheartedly. 

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