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Antibody Platform

Sheep Polyclonal Antibody Production

Introduction

Polyclonal antibodies have been widely utilized in the area of detection of pathogens. Sheep are commonly used for the generation of polyclonal antibodies, due to their convenience in handling immunization and bleeding. Polyclonal antibody production in sheep can be generated more quickly and relatively cheaply and save the amount of expertise or time in comparison with monoclonal antibody production.

 

Details

A comprehensive protocol that we developed of polyclonal antibody production includes a customizable choice for antigen preparation, immunizing sheep, collecting sera, antiserum detection, and antibodies purification. The specific service package is as follows.

Sheep polyclonal antibody preparation.png

Highlights

1. We guarantee to reach a minimum rabbit antiserum titer of 1:50,000 as measured with our ELISA assay.

2. Injections are subcutaneous as emulsions in FCA or FIA.

 

Procedure    

 

Procedure

Protocol day

Description

Control sera collection

0

Pre-immune sera (~5 mL per sheep)

Injection

1

0.50 mg antigen in FCA, ID 20 sites

1st booster

14

0.50 mg antigen in FIA, ID 20 sites

2nd booster

28

0.50 mg antigen in FIA, ID 20 sites

Serum collection

42

5 mL sera per sheep

3rd booster

56

0.50 mg antigen in FIA, ID 20 sites

Sera collection

56

5 mL sera per sheep

4th booster

70

0.50 mg antigen in FIA, ID 20 sites

5th booster

84

0.50 mg antigen in FIA, ID 20 sites

Serum collection

84

5 mL sera per sheep

6th booster

98

0.50 mg antigen in FIA, ID 20 sites

7th booster

112

0.50 mg antigen in FIA, ID 20 sites

Serum collection

112

5 mL sera per sheep

ELISA and shipping

118

ELISA titration; Verify disposition of sheeps; Plasmapheresis from one sheep (~1,000 mL sera) provided when requested by customer

 

Deliverables

22 vials crude antibody sera

2 vials: 1–2 mL (1 per sheep) test aliquots pre-immune sera

2 vials: 5 mL (1 per sheep) per vial pre-immune sera

8 vials: 1–2 mL (4 per sheep) test aliquots post-inject sera (4 times)

8 vials: 5 mL per vial post-inject sera (4 times)

1 vial: 1–2 mL test aliquot post-inject plasma (Plasmapheresis of one sheep)

1 vial: 1,000 mL bulk post-inject plasma (Plasmapheresis of one sheep)

 

More Protein Production Services

Monoclonal antibody preparation service

Antibody labeling service

Recombinant antibody expression service

Other custom services

 

Get a Quote

Abvigen is committed to providing one-stop antibody customization services from antigen synthesis to antibody manufacturing for scientific research institutions and pharmaceutical enterprises. Our technical team will rely on rich technical experience to serve you wholeheartedly.

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