Introduction
The guinea pig is widely utilized in the field of host immunity as an animal model of influenza virus research, which is highly susceptible to influenza viruses compared to mice or other animals. Typically, guinea pigs have no signs of disease and can generate antibody to clear the viruses upon infection with influenza.
Details
Choosing the guinea pigs as model, we specialize in the antigen preparation, guinea pig immunization, sera collection, and titer dectection, antibody purification and offer a full set of services. The specific service package is as follows.
Highlights
1. We guarantee to reach a minimum rabbit antiserum titer of 1:50,000 as measured with our ELISA assay.
2. Injections are subcutaneous as emulsions in FCA or FIA.
3. The number of guinea pigs can be changed according to yourself.
4. The length of the immunization schedule can be adjusted beyond the 80-day immunization protocal to meet your requirements.
Procedure
80-day immunization protocol
Procedure | Day | Description |
Pre-immune sera collection | 0 | 1 mL per guinea pig |
Injection | 1 | 100 μg antigen in FCA, SQ 4 sites |
1st booster | 21 | 50 μg antigen in FIA, SQ 4 sites |
2nd booster | 42 | 50 μg antigen in FIA, SQ 4 sites |
Test bleed | 50 | 1 mL per guinea pig |
ELISA and shipping | 60 | ELISA titration of pre-immune and test bleed |
3rd booster | 62 | 50 μg antigen in FIA, SQ 4 sites, verify disposition of animals |
Terminal bleed | 80 | Terminal bleed 10–15 mL per guinea pig |
Deliverables
15 vials crude antibody sera, including
5 vials: 0.1 mL test aliquots pre-immune sera
5 vials: 0.1 mL test bleeds post-inject sera
5 vials: 0.5-1 mL terminal bleeds
More Protein Production Services
Monoclonal antibody preparation service
Recombinant antibody expression service
Abvigen is committed to providing one-stop antibody customization services from antigen synthesis to antibody manufacturing for scientific research institutions and pharmaceutical enterprises. Our technical team will rely on rich technical experience to serve you wholeheartedly.
Online Inquiry
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