Introduction
Polyclonal antibodies (PAbs) are important reagents used in a variety of biomedical research. Rabbits are commonly used in laboratory for the production of polyclonal antibodies. Preparation of polyclonal antibodies in rabbits is a rapid, and economic method.
Details
We provide polyclonal antibody services from peptide synthesis, antigen production, antibody purification, to labeling. The specific service package is as follows.
Peptide design
2 Immunizing rabbits
Western Blot screening antigen
Reporting titer results
Antisera production
Antibody Purification (optional)
Highlights
l We guarantee to reach a minimum rabbit antiserum titer of 1:50,000 as measured with our ELISA assay. Two New Zealand White rabbits are Specific Pathogen-Free.
l Injections are subcutaneous as emulsions in FCA or FIA.
Procedure
There are three standard protocol options for immunizing rabbits, of which 70-day protocol is optimized to produce antiserum in the shortest possible time and popular among individual academic researchers.
70-day Procedure | 90-day Procedure | 120-day Procedure | ||||||
Pre-immune bleed collection | 0 | 5 mL per rabbit | Pre-immune bleed collection | 0 | 5 mL per rabbit | Pre-immune bleed collection | 0 | 5 mL per rabbit |
Immunization | 1 | 0.50 mg of antigen in FCA, 10 SQ sites | Immunization | 1 | 0.25 mg antigen in FCA, SQ 4 sites | Immunization | 1 | 0.25 mg antigen in FCA, SQ 4 sites |
1st booster | 14 | 0.25 mg of antigen in FIA, 4 SQ sites | 1st booster | 14 | 0.10 mg antigen in FIA, 4 SQ sites | 1st booster | 14 | 0.10 mg antigen in FIA, 4 SQ sites |
2nd booster | 28 | 0.25 mg of antigen in FIA, 4 SQ sites | Sera collection | 28 | 25 mL per rabbit | 2nd booster | 28 | 0.10 mg antigen in FIA, 4 SQ sites |
Sera collection | 35 | Bleed (~25 mL per rabbit) | 2nd booster | 42 | 0.10 mg antigen in FIA, 4 SQ sites | Sera collection | 42 | 25 mL per rabbit |
3rd Booster | 42 | 0.25 mg of antigen in FIA, 4 SQ sites | Sera collection | 56 | 25 mL per rabbit | 3rd booster | 56 | 0.10 mg antigen in FIA, 4 SQ sites |
Sera collection | 56, 58 | Two bleeds (~50 mL total per rabbit) | 3rd booster | 56 | 0.10 mg antigen in FIA, 4 SQ sites | Sera collection, ELISA titer | 70 | 25 mL per rabbit |
ELISA and shipping | 60 | ELISA titration ; Verify disposition of rabbits; decide to continue or terminate | Sera collection | 70, 72 | Two bleeds (~50 mL total per rabbit) | 4th booster | 84 | 0.10 mg antigen in FIA, 4 SQ sites |
ELISA and shipping | 77 | ELISA titration ; Verify disposition of rabbits; decide to continue or terminate | Sera collection, ELISA titer | 98, 100 | Two bleeds (~50 mL total per rabbit) | |||
ELISA and shipping | 106 | ELISA titration ; Verify disposition of rabbits; decide to continue or terminate | ||||||
Deliverables
l Pre-immune sera
l Antisera or purified antibodies
l Remaining antigens
More Antiboy Production Services
Monoclonal antibody preparation service
Recombinant antibody expression service
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