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Antibody Platform

Rabbit Polyclonal Antibody Production

Introduction

????1.jpgPolyclonal antibodies (PAbs) are important reagents used in a variety of biomedical research. Rabbits are commonly used in laboratory for the production of polyclonal antibodies. Preparation of polyclonal antibodies in rabbits is a rapid, and economic method.

 

Details

We provide polyclonal antibody services from peptide synthesis, antigen production, antibody purification, to labeling. The specific service package is as follows.

Peptide design

2 Immunizing rabbits

Western Blot screening antigen

Reporting titer results

Antisera production

Antibody Purification (optional)

Highlights

We guarantee to reach a minimum rabbit antiserum titer of 1:50,000 as measured with our ELISA assay. Two New Zealand White rabbits are Specific Pathogen-Free.

Injections are subcutaneous as emulsions in FCA or FIA.

 

Procedure

There are three standard protocol options for immunizing rabbits, of which 70-day protocol is optimized to produce antiserum in the shortest possible time and popular among individual academic researchers.

70-day Procedure

90-day Procedure

120-day Procedure

Pre-immune bleed collection

0

5 mL per rabbit

Pre-immune bleed collection

0

5 mL per rabbit

Pre-immune bleed collection

0

5 mL per rabbit

Immunization

1

0.50 mg of antigen in FCA, 10 SQ sites

Immunization

1

 0.25 mg antigen in FCA, SQ 4 sites

Immunization

1

0.25 mg antigen in FCA, SQ 4 sites

1st booster

14

0.25 mg of antigen in FIA, 4 SQ sites

1st booster

14

 0.10 mg antigen in FIA, 4 SQ sites

1st booster

14

0.10 mg antigen in FIA, 4 SQ sites

2nd booster

28

 0.25 mg of antigen in FIA, 4 SQ sites

Sera collection

28

25 mL per rabbit

2nd booster

28

0.10 mg antigen in FIA, 4 SQ sites

Sera collection

35

Bleed (~25 mL per rabbit)

2nd booster

42

0.10 mg antigen in FIA, 4 SQ sites

Sera collection

42

25 mL per rabbit

3rd Booster

42

0.25 mg of antigen in FIA, 4 SQ sites

Sera collection

56

25 mL per rabbit

3rd booster

56

0.10 mg antigen in FIA, 4 SQ sites

Sera collection

56, 58

Two bleeds (~50 mL total per rabbit)

3rd booster

56

 0.10 mg antigen in FIA, 4 SQ sites

Sera collection, ELISA titer

70

25 mL per rabbit

ELISA and shipping

60

ELISA titration ;  Verify disposition of rabbits; decide to continue or terminate

Sera collection

70, 72

Two bleeds (~50 mL total per rabbit)

4th booster

84

 0.10 mg antigen in FIA, 4 SQ sites




ELISA and shipping

77

ELISA titration ;  Verify disposition of rabbits; decide to continue or terminate

Sera collection, ELISA titer

98, 100

Two bleeds (~50 mL total per rabbit)







ELISA and shipping

106

ELISA titration ;  Verify disposition of rabbits; decide to continue or terminate

Deliverables 

Pre-immune sera

Antisera or purified antibodies

Remaining antigens

 

More Antiboy Production Services

Monoclonal antibody preparation service

Antibody labeling service

Recombinant antibody expression service

Other custom services

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