Introduction
Polyclonal antibodies (PAbs) are important reagents used in a variety of biomedical research. Rabbits are commonly used in laboratory for the production of polyclonal antibodies. Preparation of polyclonal antibodies in rabbits is a rapid, and economic method.
Details
We provide polyclonal antibody services from peptide synthesis, antigen production, antibody purification, to labeling. The specific service package is as follows.
Peptide design
2 Immunizing rabbits
Western Blot screening antigen
Reporting titer results
Antisera production
Antibody Purification (optional)
Highlights
l We guarantee to reach a minimum rabbit antiserum titer of 1:50,000 as measured with our ELISA assay. Two New Zealand White rabbits are Specific Pathogen-Free.
l Injections are subcutaneous as emulsions in FCA or FIA.
Procedure
There are three standard protocol options for immunizing rabbits, of which 70-day protocol is optimized to produce antiserum in the shortest possible time and popular among individual academic researchers.
70-day Procedure | 90-day Procedure | 120-day Procedure | ||||||
Pre-immune bleed collection | 0 | 5 mL per rabbit | Pre-immune bleed collection | 0 | 5 mL per rabbit | Pre-immune bleed collection | 0 | 5 mL per rabbit |
Immunization | 1 | 0.50 mg of antigen in FCA, 10 SQ sites | Immunization | 1 | 0.25 mg antigen in FCA, SQ 4 sites | Immunization | 1 | 0.25 mg antigen in FCA, SQ 4 sites |
1st booster | 14 | 0.25 mg of antigen in FIA, 4 SQ sites | 1st booster | 14 | 0.10 mg antigen in FIA, 4 SQ sites | 1st booster | 14 | 0.10 mg antigen in FIA, 4 SQ sites |
2nd booster | 28 | 0.25 mg of antigen in FIA, 4 SQ sites | Sera collection | 28 | 25 mL per rabbit | 2nd booster | 28 | 0.10 mg antigen in FIA, 4 SQ sites |
Sera collection | 35 | Bleed (~25 mL per rabbit) | 2nd booster | 42 | 0.10 mg antigen in FIA, 4 SQ sites | Sera collection | 42 | 25 mL per rabbit |
3rd Booster | 42 | 0.25 mg of antigen in FIA, 4 SQ sites | Sera collection | 56 | 25 mL per rabbit | 3rd booster | 56 | 0.10 mg antigen in FIA, 4 SQ sites |
Sera collection | 56, 58 | Two bleeds (~50 mL total per rabbit) | 3rd booster | 56 | 0.10 mg antigen in FIA, 4 SQ sites | Sera collection, ELISA titer | 70 | 25 mL per rabbit |
ELISA and shipping | 60 | ELISA titration ; Verify disposition of rabbits; decide to continue or terminate | Sera collection | 70, 72 | Two bleeds (~50 mL total per rabbit) | 4th booster | 84 | 0.10 mg antigen in FIA, 4 SQ sites |
ELISA and shipping | 77 | ELISA titration ; Verify disposition of rabbits; decide to continue or terminate | Sera collection, ELISA titer | 98, 100 | Two bleeds (~50 mL total per rabbit) | |||
ELISA and shipping | 106 | ELISA titration ; Verify disposition of rabbits; decide to continue or terminate | ||||||
Deliverables
l Pre-immune sera
l Antisera or purified antibodies
l Remaining antigens
More Antiboy Production Services
Monoclonal antibody preparation service
Recombinant antibody expression service
Online Inquiry
To learn more about information protection, please see Privacy Policy. If you have any questions about CRO service, please email at info@abvigenus.com or call 929-202-3014
