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Mouse Monoclonal Antibody Production Services

Introduction


Monoclonal antibodies are highly specific antibodies raised from a single B cell clone that are specific to a particular epitope, and are typically prepared using hybridoma technology. Monoclonal antibodies are characterized by specificity, homogeneity, high efficiency and unlimited supply. It shows great vitality in the diagnosis, prevention and treatment of diseases, the basic research and clinical medicine in the fields of immunology, medicine, biology, etc.

 

With its advanced and mature technology platform, excellent management team and experienced technical research and development team, Abvigen Inc has become the leading brand of antibody preparation in the industry.

 

Description

1 antigen

 

A Customer provide antigen

 

Protein or polypeptide fragment: >10mg, purity >85%, concentration >1mg/ml

 

B Preparation of antigen

 

Peptide synthesis, coupling (MS/HPLC)

 

Recombinant protein expression (SDS-PAGE)

 

2 Immunization

 

6-8 weeks female BALB/c mice were immunized with the antigen following a pre-established immunization protocol to produce sensitized B lymphocytes in mice.

 

3 Cell fusion

 

Lymphocytes fuse with myeloma cells to form hybridoma cells by polyethylene glycol.

 

4 Screening

 

Hybridoma cells in HAT medium were cultured by limiting dilution method. Positive hybridoma cells capable of producing the desired monoclonal antibody are selected.

 

5 Clone

 

Cloning and amplification of selected positive hybridoma cells

 

6 Preparation

 

In vivo induction

 

BALB/c mice were intraperitoneally inoculated with hybridoma cells for 1-2 weeks. Ascites was taken to obtain a large number of monoclonal antibodies.

 

In vitro culture

 

Monoclonal antibody is produced and secreted from culture flask. The culture supernatant is collected to obtain the desired monoclonal antibody.

 

In vitro culture

 

The culture flask is cultured, the monoclonal antibody is produced and secreted, and the culture supernatant is collected to obtain the desired monoclonal antibody.

 

Advantages

 

Personalized service according to customer's specific requirements

 

Professional antibody screening and purification technology

 

Fast delivery: the production cycle of the monoclonal antibody only takes 4-6 months

 

One-stop service from immunogen preparation to antibody sequencing


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