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Yeast Protein Expression Services

Introduction

 

Prokaryotic expression is mature and easy to operate. But for eukaryotes' proteins, misfolding often occurs due to glycosylation, folding partners, etc. The yeast expression system is the most cost-effective eukaryotic protein expression system, which can successfully achieve intracellular expression or secretory expression with its relatively inexpensive amplification medium, easier culture conditions, suitable for industrial amplification, and effectively reducing production costs. It is a powerful tool for expressing large-scale proteins. Yeast protein expression can be used to prepare protein products with high added value close to native proteins.

 

Abvigen Inc's yeast protein expression system (S. cescerevisiae and P. pastoris) is an important component of the recombinant protein expression platform. Abvigen Inc can provide gene sequence optimization, multi-vector selection construction, multi-strain transformation, large-scale production, and provide customer with high-purity soluble protein.

 

Description

 

1 Gene synthesis and codon optimization (optional)

 

2 Vector construction

 

Sequence analysis according to customer needs

 

Cloning into the expression vector

Plasmid sequencing

Large amount of plasmid preparation

Cycle: 2-3 weeks

 

3 Vector linearization and transformation

 

Select appropriate endonuclease for linearizing recombinant plasmid

 

Transferred to a suitable yeast cell by electroporation

 

Screen for high copy expression strains by PCR

 

Screen positive clones for test

 

Cycle: 1-2 weeks

 

4 Expression and purification

 

Optimize strain culture conditions

 

SDS-PAGE/WB

 

Cycle: 1-2 weeks

 

5 Large amount of expression and purification (optional)

 

Cycle: 2-3 weeks

 

6 Protein post processing (optional)

 

Label excision, endotoxin removal, lyophilization, N-terminal sequencing, etc.

 

Advantages

 

Efficient expression vector, high expression level: S. cescerevisiae and P. pastoris expression system for high success rate of protein expression

 

One-stop service: gene design and optimization, optimization of protein expression conditions, construction of constitutive expression strains, protein purification and modification, data analysis and report processing

 

Large-scale protein expression production


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